Anti-OXSR1 Antibody Picoband™

Product Name

Anti-OXSR1 Antibody Picoband™

View all OXSR1 Antibodies

SKU/Catalog Number

A05141-2

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-OXSR1 Antibody Picoband™ catalog # A10973-2. Tested in WB, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-OXSR1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05141-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Clone Number

1B9

Isotype

IgG

Immunogen

E.coli-derived human OXSR1 recombinant protein (Position: K351-S527). Human OXSR1 shares 89.8% and 85.4% amino acid (aa) sequence identity with mouse and rat OXSR1, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A05141-2 is reactive to OXSR1 in Human, Mouse, Rat

Applications

A05141-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

58 kDa

Calculated molecular weight

58.022kDa

Background of OXSR1

Serine/threonine-protein kinase OSR1 is an enzyme that in humans is encoded by the OXSR1 gene. The product of this gene belongs to the Ser/Thr protein kinase family of proteins. It regulates downstream kinases in response to environmental stress, and may play a role in regulating the actin cytoskeleton.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25 µg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of OXSR1 using anti-OXSR1 antibody (A05141-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human U251 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat testis tissue lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OXSR1 antigen affinity purified polyclonal antibody (Catalog # A05141-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OXSR1 at approximately 58 kDa. The expected band size for OXSR1 is at 58 kDa.

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Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-OXSR1 antibody (A05141-2).
Overlay histogram showing MCF-7 cells stained with A05141-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OXSR1 Antibody (A05141-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For OXSR1 (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.7.11; EC 2.7.11.1; KIAA1101OSR1serine/threonine-protein kinase OSR1; Oxidative stress-responsive 1 protein; oxidative-stress responsive 1 OXSR1 OSR1 oxidative stress responsive kinase 1 serine/threonine-protein kinase OSR1|oxidative stress responsive 1|oxidative stress-responsive 1 protein

For more info on OXSR1, check out the OXSR1 Infographic

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In this infographic, you will see the following information for OXSR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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